Literature summary extracted from
Saxl, R.L.; Anand, G.S.; Stock, A.M.
Synthesis and Biochemical Characterization of a Phosphorylated Analogue of the Response Regulator CheB (2001), Biochemistry, 40, 12896-12903.
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.1.1.61 |
C207S/C309S |
wild-type and mutant enzyme show significantly greater activity than the unphosphorylated proteins |
Salmonella enterica subsp. enterica serovar Typhimurium |
3.1.1.61 |
D56C/C207S/C309S |
the half-life of mutant-SPO3 is 28 days, the unphosphorylated wild-type and mutant enzyme show the same enzyme activities |
Salmonella enterica subsp. enterica serovar Typhimurium |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.1.61 |
Salmonella enterica subsp. enterica serovar Typhimurium |
- |
- |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
3.1.1.61 |
phosphoprotein |
enzyme is activated in vivo by phosphorylation of a single aspartate, Asp56, in its regulatory domain |
Salmonella enterica subsp. enterica serovar Typhimurium |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
3.1.1.61 |
protein L-glutamate O5-methyl ester + H2O = protein L-glutamate + methanol |
the enzyme is a response regulator protein in the bacterial chemotaxis two-component signal transduction pathway |
Salmonella enterica subsp. enterica serovar Typhimurium |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.1.1.61 |
More |
the CheB protein is composed of at least 2 structurally distinct proportions: a C-terminal catalytic domain, and a N-terminal region which modulates esterase activity |
Salmonella enterica subsp. enterica serovar Typhimurium |